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4:18
thermofisher.com
Optimize qPCR Using SYBR Green Assays - Ask TaqMan #38
Are you new to using SYBR Green Assays for qPCR or having trouble getting accurate results? Today, let’s discuss how you can design and optimize qPCR using SYBR Green assays as the detection method. First, beware of reverse transcription (RT) bias when converting RNA to cDNA. Nearly all RT enzymes have the potential to introduce
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