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Biovision on Instagram: "🧬 DNA Extraction Protocol with the Wonderful Wiragen Kit @wiragen.sarl ✨ 🧪 Sample type: Heart tissue (25–50 mg) 📍 Tube: 1.5 mL sterile microcentrifuge tube 🔹 1. Sample Preparation and Lysis 1️⃣ Add 25–50 mg of tissue sample into a 1.5 mL microcentrifuge tube. 2️⃣ Add 200 μL of Lysis Buffer (LBB5) (from the Wiragen kit). 3️⃣ Add 20 μL of Proteinase K solution (20 mg/mL) (from the Wiragen kit). 4️⃣ Mix immediately 🔄 and incubate at +70°C 🌡️ for 1 hour, vortexing occa
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Biovision on Instagram: "🧬 DNA Extraction Protocol with the Wonderful Wiragen Kit ✨ 🧪 Sample type: Liver tissue (25–50 mg) 📍 Tube: 1.5 mL sterile microcentrifuge tube 🔹 1. Sample Preparation and Lysis 1️⃣ Add 25–50 mg of liver tissue sample into a 1.5 mL microcentrifuge tube. 2️⃣ Add 200 μL of Lysis Buffer (LBB5) (from the Wiragen kit). 3️⃣ Add 20 μL of Proteinase K solution (20 mg/mL) (from the Wiragen kit). 4️⃣ Mix immediately 🔄 and incubate at +70°C 🌡️ for 1 hour, vortexing occasionally
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